准教授 小林 直樹

プロフィール

2002年東京工業大学・生命理工学部卒業、2004年東京工業大学大学院・生命理工学研究科修士課程修了、2007年同大学院博士課程修了。学位（理学博士）取得後、東京工業大学大学院および国立の衛生研究所で博士研究員として勤務。2015年4月に麻布大学に講師として着任。2021年4月より現職。

生物の形質（姿かたち、行動、性格、毒性など）はそれぞれの生物の遺伝子もしくは遺伝子の使われ方によって決まります。食品を汚染する危害性微生物についても、その危害性（形質）を生み出す遺伝的要因があります。危害性に関わる遺伝子レベルのメカニズムを詳細に調べることで、食品を汚染する微生物の制御に、そして食の安全に貢献する研究を進めます。

近年、食品等の微生物検査においても迅速で簡便な遺伝子レベルでの検査が主流になりつつあります。遺伝子に関わる技術を身に着けたいという人は、食品安全科学研究室を一度のぞいてみてください。

研究業績

英 文

1. Oshiro N, Nagasawa H, Nishimura M, Kuniyoshi K, Kobayashi N, Sugita-Konishi Y, Ikehara T, Tachihara K, Yasumoto T. Analytical studies on ciguateric fish in Okinawa, Japan (II): The grouper Variola albimarginata. J Mar Sci Eng. 11(2): 242, 2023
2. Oshikata C, Watanabe M, Hashimoto K, Yamazaki A, Kobayashi N, Konuma R, Ishida M, Kobayashi S, Shimada T, Kaneko T, Kamata Y, Kuriyama S, Kure S, Yanai M, Tsurikisawa N. Mite allergen avoidance decreases allergic symptoms in children in Ishinomaki city of Japan after natural disasters. Allergol Immunopathol (Madr), 50(2): 23-32, 2022 doi: 10.15586/aei.v50i2.483.
3. Oonaka K#, Kobayashi N#, Uchiyama Y, Honda M, Miyake S, Sugita-Konishi Y. In vivo and In vitro Mitigation Effects of Lactic Acid Bacteria Derived from Fresh Vegetables on Aflatoxins. Shokuhin Eiseigaku Zasshi. 2021;62(5):148-156. doi: 10.3358/shokueishi.62.148. PMID: 34732640. #Contributed equally
4. Oshikata C, Watanabe M, Ishida M, Kobayashi S, Hashimoto K, Kobayashi N, Yamazaki A, Konuma R, Shimada T, Kaneko T, Kamata Y, Kuriyama S, Yanai M, Tsurikisawa N. Mite avoidance decreased mite-specific IgE levels and ameliorated asthma symptoms in subjects who lived in temporary housing after natural disasters. Allergol. Immunopathol. (Madr), 49: 171-179, 2021
5. Oshikata C, Watanabe M, Ishida M, Kobayashi S, Hashimoto K, Kobayashi N, Yamazaki A, Konuma R, Kaneko T, Kamata Y, Kuriyama S, Yanai M, Tsurikisawa N. Association between Temporary Housing Habitation after the 2011 Japan Earthquake and Mite Allergen Sensitization and Asthma Development. Int. Arch. Allergy Immunol., 1-13, 2021
6. Watanabe M, Konuma R, Kobayashi N, Yamazaki A, Kamata Y, Hasegawa K, Kimura N, Tsurikisawa N, Oshikata C, Sugita-Konishi Y, Takatori K, Yoshino H, Hara-Kudo Y. Indoor Fungal Contamination in Temporary Housing after the East Japan Great Earthquake Disaster. Int. J. Environ. Res. public health, 18: 3296, 2021
7. Oshiro N, Nagasawa H, Kuniyoshi K, Kobayashi N, Sugita-Konishi Y, Asakura H, Yasumoto T. Characteristic Distribution of Ciguatoxins in the Edible Parts of a Grouper, Variola louti. Toxins, 13: 218, 2021
8. Yoshinami M, Machida Rikuya, Kobayashi N, Sugita-Konishi Y, Furuhata K. Multifaceted fungal characteristics determining the fungal feeding preferences of the psocid, Liposcelis bostrychophila badonnel (Psocoptera: Liposcelidae). J. Stored Prod. Res., 88: 101659, 2020
9. Aoki W, Watanabe M, Watanabe M, Kobayashi N, Terajima J, Sugita-Konishi Y, Kondo K, Hara-kudo Y. Discrimination between edible and poisonous mushrooms among Japanese Entoloma sarcopum and related species based on phylogenetic analysis and insertion/deletion patterns of nucleotide sequences of the cytochrome oxidase 1 gene. Genes Genet. Sys., 95: 133-139, 2020
10. Izawa K, Kubosaki A, Kobayashi N, Akiyama Y, Yamazaki A, Hashimoto K, Konuma R, Kamata Y, Hara-Kudo Y, Hasegawa K, Ikaga T, Watanabe M. Comprehensive fungal community analysis of house dust using next-generation sequencing. Int. J. Environ. Res. Public Health, 17: 5842, 2020
11. Yamazaki A, Hiroshima T, Yamaguchi Y, Shirafuji Y, Taira K, Saito M, Kobayashi N, Sugita-Konishi Y, Kamata Y. Presence of Sarcocystis sybillensis in Japanese sika deer (Cervus nippon) captured in its native territory and its phylogenetic relationship with Sarcocystis nipponi. Parasitol. Res., 119: 2309-2315, 2020
12. Okano T#, Kobayashi N#, Izawa K, Yoshinari T, Sugita-Konishi Y. Whole Genome Analysis Revealed the Genes Responsible for Citreoviridin Biosynthesis in Penicillium citreonigrum. Toxins, 12: 125, 2020 #Contributed equally
13. Kubosaki A, Kobayashi N, Watanabe M, Yoshinari T, Takatori K, Kikuchi Y, Hara-Kudo Y, Terajima J, Sugita-Konishi Y. A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase. Biocontrol Sci., 25: 113-118, 2020
14. Onami J, Kobayashi N, Watanabe M, Yamada O, Mizutani O, Yokoyama K, Takahashi H, Chibana H, Kamata Y. An updated data portal for fungal allergens with curated information. Bioinformation, 15: 820-823, 2019
15. Maragos CM, Uchiyama Y, Kobayashi N, Kominato F, Sugita-Konishi. Development and Characterization of Monoclonal Antibodies for the Mycotoxin Citreoviridin. Toxins, 11: 630, 2019
16. Uchiyama Y, Takino M, Noguchi M, Shiratori N, Kobayashi N, Sugita-konishi. The In Vivo and In Vitro Toxicokinetics of Citreoviridin Extracted from Penicillium citreonigrum. Toxins, 11: e360, 2019
17. Sugita-Konishi Y#, Kobayashi N#, Takasaki K, Kanno T, Itoh M, Riztyan, Futo S, Asakura H, Taira K, Kawakami Y. Detection of Sarcocystis spp. and Shiga toxin-producing Escherichia coli in Japanese sika deer meat using a loop-mediated isothermal amplification-lateral flow strip. J. Vet. Med. Sci., 81: 586-592, 2019 #Contributed equally
18. Kobayashi N, Sakurai K, Nakarai R, Shigaki K, Horikawa K, Honda M, Sugiura Y, Watanabe M, Takino M, Sugita-Konishi Y. Microflora of Mycotoxigenic Fungi in Rice Grains in Kyushu Region of Japan and Their Changes during Storage under non-Controlled Conditions. Biocontrol. Sci., 24: 161-166, 2019
19. Honda M, Sawaya M, Taira K, Yamazaki A, Kamata Y, Shimizu H, Kobayashi N, Sakata R, Asakura H, Sugita-Konishi Y. Effects of temperature, pH and curing on the viability of Sarcocystis, a Japanese sika deer (Cervus Nippon centralis) parasite, and the inactivation of their diarrheal toxin. J. Vet. Med. Sci., 80: 1337-1344. 2018
20. Onami J, Watanabe M, Yoshinari T, Hashimoto R, Kitayama M, Kobayashi N, Sugita-Konishi Y, Kamata Y, Takahashi H, Kawakami H, Terajima J. Fumonisin-production by Aspergillus section Nigri isolates from Japanese Foods and Environments. Food saf., 6: 74-82, 2018
21. Kobayashi N, Kubosaki A, Takahashi Y, Yanai M, Konuma R, Uehara S, Chiba T, Watanabe M, Terajima J, Sugita-Konishi Y. Distribution of sterigmatocystin-producing Aspergilli in Japan. Food Saf., 6: 67-73, 2018
22. Yamazaki A, Honda M, Kobayashi N, Ishizaki N, Asakura H, Sugita-Konishi Y. The sensitivity of commercial kits in detecting the genes of pathogenic bacteria in venison. J. Vet. Med. Sci., 80: 706-709. 2018
23. Yoshinami M, Kobayashi N, Takino M, Sugita-Konishi Y. Isolation of fungi from a fungivorous insect, the minute brown scavenger beetle (Latridiidae), and their potential ability for mycotoxin production. JSM Mycotoxins, 68: 7-11, 2018
24. Shiratori N#, Kobayashi N#, Tulayakul P, Sugiura Y, Takino M, Endo O, Sugita-Konishi Y. Occurrence of Penicillium brocae and Penicillium citreonigrum, related to mutagenic and toxic metabolites, respectively, in commercially available rice grains of Thailand. Toxins, 9: E194, 2017 # Equally contributed
25. Nishihara H#, Kobayashi N#, Kimura-Yoshida C, Yan K, Bormuth O, Ding Q, Nakanishi A, Sasaki T, Hirakawa M, Sumiyama K, Furuta Y, Tarabykin V, Matsuo I, Okada N. Coordinately co-opted multiple transposable elements constitute an enhancer for wnt5a expression in the mammalian secondary palate. PLoS Genet., 12: e1006380, 2016 # Equally contributed
26. Kobayashi N, Maeda E, Saito S, Furukawa I, Ohnishi T, Watanabe M, Terajima J, Hara-Kudo Y. Association of cell-adhesion activities with virulence in shiga toxin-producing Escherichia coli O103：H2. Biocontrol. Sci., 21: 57-61, 2016
27. Kobayashi N, Lee K, Yamazaki A, Saito S, Furukawa I, Kono T, Maeda E, Isobe J, Sugita-Konishi Y, Hara-Kudo Y. Virulence gene profiles and population genetic analysis for exploration of pathogenic serogroups of Shiga toxin-producing Escherichia coli. J. Clin. Microbiol., 51: 4022-8, 2013
28. Kuroiwa A, Terai Y, Kobayashi N, Yoshida K, Suzuki M, Nakanishi A, Matsuda Y, Watanabe M, Okada N. Construction of chromosome markers from the Lake Victoria cichlid Paralabidochromis chilotes and their application to comparative mapping. Cytogenet. Genome Res., 142: 112-20, 2014
29. Lee K, Kobayashi N, Watanabe M, Sugita-Konishi Y, Tsubone H, Kumagai S, Hara-Kudo Y. Spread and change in stress resistance of Shiga toxin-producing Escherichia coli O157 on fungal colonies. Microb. Biotechnol., 7: 621-9, 2013
30. Nakanishi A, Kobayashi N, Suzuki-Hirano A, Nishihara H, Sasaki T, Hirakawa M, Sumiyama K, Shimogori T, Okada N. A SINE-derived element constitutes a unique modular enhancer for mammalian diencephalic Fgf8. PLoS One, 7: e43785, 2012
31. Kobayashi N, Watanabe M, Hara-Kudo Y. Distinctive identification of Cladosporium sphaerospermum and Cladosporium halotolerans based on physiological methods. J. Syst. Evol., 50: 235-43, 2012
32. Tashiro K#, Teissier A#, Kobayashi N#, Nakanishi A, Sasaki T, Yan K, Tarabykin V, Vigier L, Sumiyama K, Hirakawa M, Nishihara H, Pierani A, Okada N. A mammalian conserved element derived from SINE displays enhancer properties recapitulating Satb2 expression in early-born callosal projection neurons. PLoS One, 6: e28497, 2011 # Equally contributed
33. Kobayashi N, Watanabe M, Horiike T, Kohara Y, Okada N. Extensive analysis of EST sequences reveals that all cichlid species in Lake Victoria share almost identical transcript sets. Gene, 441: 187-91, 2009
34. Kobayashi N, Watanabe M, Kijimoto T, Fujimura K, Nakazawa M, Ikeo K, Kohara Y, Gojobori T, Okada N. magp4 gene may contribute to the diversification of cichlid morphs and their speciation. Gene, 373: 126-33, 2006
35. Watanabe M, Kobayashi N, Shin-i T, Horiike T, Tateno Y, Kohara Y, Okada N. Extensive analysis of ORF sequences from two different cichlid species in Lake Victoria provides molecular evidence for a recent radiation event of the Victoria species flock. identity of EST sequences between Haplochromis chilotes and Haplochromis sp. "Redtailsheller". Gene, 343: 263-9, 2004
36. Watanabe M, Kobayashi N, Fujiyama A, Okada N. Construction of a BAC library for Haplochromis chilotes, a cichlid fish from Lake Victoria. Genes Genet. Syst., 78: 103-5 2003

和 文

1. 内山 陽介, 山崎 大樹, 小林 直樹, 諫田 泰成, 小西 良子 . ヒトiPS細胞由来心筋細胞に対するカビ毒シトレオビリジンの電気生理学的影響. 食品衛生学雑誌, 63: 210-217, 2022
2. 長沢 寛弥, 國吉 杏子, 谷川 敏明, 小林 直樹, 小西 良子, 朝倉 宏, 大城 直雅. 小笠原群島産バラハタVariola loutiのシガトキシン類分析. 食品衛生学雑誌, 62: 157-161, 2021
3. 大塚佳代子， 小林直樹， 森田幸雄， 宮坂次郎， 和栗敦， 楠原一， 工藤由起子. 焼肉調理における腸管出血性大腸菌の生残の解析. 日本食品衛生学雑誌, 55: 79-87, 2014
4. 小林直樹. 黒麹菌を材料としたカビアレルゲン性評価への取り組み. JSM Mycotoxins, 65: 115-20, 2015
5. 小林直樹, 工藤由起子, 寺嶋淳. 腸管出血性大腸菌. 臨床と微生物, 41: 27-31, 2014
6. 小林直樹*, 工藤由起子. 腸管出血性大腸菌の分子生物学的研究と食品での検査法の発展. 日本食品微生物学会雑誌, 30: 147-55, 2013
7. 早川亮太, 小林直樹, 加藤登, 工藤由起子, 荒木惠美子. 日本産海産魚におけるヒスタミン生成魚種および凍結保存によるヒスタミン生成の抑制低減の検討. 日本食品衛生学雑誌, 54: 402-9, 2013

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